![]() ![]() These steps often include adjusting brightness and contrast, removing noise, and picking a color scheme that is most visible to the human eye. Data AnalysisĪfter acquisition, most images will benefit from additional post-processing. You may also bring your own portable storage device. Double-click the "Sync Data" icon (located next to the "Data" folder) to upload your data to the PIF FTP site. The facility is not responsible for lost data. Data found in any other location will be deleted immediately and without notice. Only save data in your folder that is within the main Data folder. Upon acquisition, all data is saved within the "Data" folder on the acquisition computer's desktop. PMOD also supports "screenshots" or movies of the 3D visualizations, which are stored as JPGs. From there, the data can be converted to a sequence of many of kinds of 2D image formats, including TIFF. This file format can be opened directly in ImageJ or PMOD. ![]() ![]() SPECT/CT: After reconstruction, 3D SPECT and CT images are output as 16-bit NIfTI format files (*.nii). (Note that 12-bit color images cannot be opened in standard Windows software or MS Office. It is also possible to capture screen shots by pressing "x" on the keyboard. If you prefer to process your data using other software, it can be easily exported as a TIFF or other common image formats. This file type can only be opened in Nikon's Elements software, the free (but limited) Nikon Elements ND2 Viewer, or in Image J using the ND2 Reader Plugin. This format stores all n-dimensions of data in a single file as well as the acquisition settings and other metadata. We highly recommend that all data is saved in Nikon's proprietary *.nd2 (or *.ics) format. Interested in a 2 credit Graduate level lecture course in microscopy here at Purdue? Find out moreīIOL 69500 - Microsopy For Life Scientists Data Management Data Formatting RETURN TO RESEARCH Reopening Town Hall Graduate Student Course ![]()
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